Protein_Domain

Part:BBa_K203000:Design

Designed by: Douaa Mugahid and Michael Bartoschek   Group: iGEM09_Heidelberg   (2009-10-15)

Plasma membrane targeting (GPI anchor)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 146
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 146
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 146
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

-This part was isolated from a plasmid provided by the [http://www.bioquant.uni-heidelberg.de/research/groups/screening_of_cellular_networks/home.html Starkuviene lab], BioQuant, Heidelberg, Germany.

-This part was made to be fused N-terminally to other protein domains, and thus lacks a stop codon.

-This part is BBb compatible and is thus suitable for use for protein fusion with other BBb-compatible parts.

Source

Plasmid DNA provided by [http://www.bioquant.uni-heidelberg.de/research/groups/screening_of_cellular_networks/home.html Starkuviene lab], BioQuant, Heidelberg, Germany.

References